标题：
The role of stem cell transcription factors in new bone formation in patients with ankylosing spondylitis
讲者：
金茜玫
单位：
第二军医大学附属长征医院
播放：
2143
论文摘要：
Objectives：The aim of this research is to figure out the role of stem cell transcription factors（OCT-4, SOX-2, c-MYC, KLF-4）in ectopic ossification and to better understand the pathogenesis of new bone formation in AS patients.
Methods: Ligament tissues were collected from hip joints from AS patients and control groups who had the operation of hip replacement. The expression levels of OCT-4, SOX-2, c-MYC, KLF-4 were analyzed via real-time polymerase chain reaction (RT-PCR), Western blot (WB) and immunohistochemistry (IHC).
Results: We found that OCT-4, SOX-2 and c-MYC were higher expressed in AS ligament than controls. More interestingly, during the process of inducing primary fibroblast to osteoblast, OCT-4 increased at first several days and then decreased gradually, but c-MYC was always higher expressed than controls. These results suggest that fibroblast may reprogram itself during this process. We also used cytokines to stimulate fibroblasts and found out that OCT-4, SOX-2 and c-MYC can be upregulated by IL-23 alone.
Conclusions: Our results indicated the critical role of the stem cell transcription factors in the process of fibroblast ossification. Furthermore, we put forward a hypothesis that after a long term of inflammatory cytokine stimulation, fibroblast could dedifferentiate to a pluripotent state and then re-differentiate to osteoblast to participate new bone formation in AS.
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