标题：
骨桥蛋白对高血压血管钙化患者的巨噬细胞调节作用
讲者：
葛茜
单位：
上海交通大学医学院附属瑞金医院
播放：
2380
论文摘要：
Vascular calcification (VC) is a highly regulated ectopic mineral deposition process involving immune cell infiltration in vasculature, and
promoted by hypertension. The matricellular glycoprotein osteopontin(OPN) is strongly induced in myeloid cells as a potential mediator of vascular injury. This study aims to examine whether OPN is involved in the regulation of macrophage activation and osteoclast formation in hypertensive subjects with VC. We detected an increased proportion of CD11c+CD163- pro-inflammatory peripheral monocytes in hypertensive subjects with VC compared to those without VC by flow cytometric analysis. Quantitative polymerase chain reaction also showed an altered expression profile of inflammatory factors in primary cultured macrophages from hypertensive subjects with VC. The hypertensive patients with VC had higher serum OPN level. Exogenous OPN promoted the differentiation of peripheral monocytes into an alternative, anti-inflammatory phenotype, and inhibited macrophage-to-osteoclast differentiation from these VC patients. In addition, calcified vessels showed an
increased osteoclasts accumulation and decreased macrophages infiltration in the of hypertensive subjects. This study demonstrated that OPN exerts an important role in the monocytes/macrophage phenotypic differentiation from hypertensive patients with VC, which includes reducing
inflammatory factor expression and attenuating osteoclast formation.
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