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张湘燕:Predominant Characteristics of ESBL-producing Escherichia coli Isolates from Patients with Pneumonia in Guizhou Province in China PPT讲座视频 中华医学会呼吸病学年会-2017(第十八次全国呼吸病学学术会议)
标题: Predominant Characteristics of ESBL-producing Escherichia coli Isolates from Patients with Pneumonia in Guizhou Province in China
讲者: 张湘燕
单位: 贵州省人民医院
播放: 1194
论文摘要: Objective: The aim of the investigation was to target analysis the predominant characteristics of ESBL-producing Escherichia coli (E. coli) strains from pneumonia patients in Guizhou province.
Methods: From January 2016 to February 2017, 69 of 237 E. coli strains producing ESBL from pneumonia patients were collected from four tertiary hospitals from Guizhou province. Phenotypic methods and antibiotic susceptibility tests were employed to characterize 69 ESBL-producers. The genotype of ESBL were analysed by amplification of PCR, direct sequencing with various set of PCR primers and the basic local alignment search tool online. Sample cluster analysis of the detection results was also performed.
Results: Among the 69 analyzed isolates, 69 (100%) harbored blaCTX-M, with in which the most predominant ESBL gene was CTX-M-14 (49/69, 71%), followed by CTX-M-15(17/69, 25%) and CTX-M-27 (15/27, 22%). Also, 61 (88%) harbored blaSHV and only 1 isolates carried blaTEM-1. More than one ESBL combination was detected in 43 isolates (62%). Forty-nine (71%) isolates demonstrated the multidrug resistant (MDR) phenotype. The sample cluster analysis showed that the multiple clone spread existed in this group of E. coli.
Conclusion: blaCTX-M is the most prevalent ESBL gene in ESBL-producing E. coli clinical isolates in Guizhou province and the dominant genotypes is blaCTX-M-14. The increasing prevalence of E. coli isolates with MDR phenotype contributes to clinical challenges and the consequent surveillance of ESBL-producing organisms is of paramount importance.
 

呼吸内科

贵州省人民医院

贵州省人民医院、贵州省呼吸疾病研究所

二级教授、主任医师、博士生导师、省核心专家

国家卫生突出贡献中青年专家、国务院政府特殊津贴专家

贵州省新冠肺炎疫情医疗救治专家组组长

健康中国推进行动计划专家咨询委员会委员

中国医师协会第四届理事会常务理事

中华医学会第25、26届理事会理事

贵州省医学会呼吸病学分会主任委员

贵州省呼吸医疗质控中心主任

主要研究方向:呼吸系统感染与危重症疾病救治等

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