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刘玲英:Human Umbilical Cord Mesenchymal Stem Cells transplantation promotes Cutaneous Wound Healing of Severe Burned Rats PPT讲座视频 中华医学会烧伤外科学分会2017年学术年会暨第二十五届全军烧伤外科学专业学术会议
标题: Human Umbilical Cord Mesenchymal Stem Cells transplantation promotes Cutaneous Wound Healing of Severe Burned Rats
讲者: 刘玲英
单位: 解放军总医院第一附属医院
播放: 1609
论文摘要: Abstract
BACKGROUND: Severe burns are a common and highly lethal trauma. The key step for severe burn therapy is to promote the wound healing as early as possible, and reports indicate that mesenchymal stem cell (MSC) therapy contributes to facilitate wound healing. In this study, we investigated effect of human umbilical cord MSCs (hUC-MSCs) could on wound healing in a rat model of severe burn and its potential mechanism. METHODS: Adult male Wistar rats were randomly divided into sham, burn, and burn transplanted hUC-MSCs. GFP labeled hUC-MSCs or PBS was intravenous injected into respective groups. The rate of wound closure was evaluated by Image Pro Plus. GFP-labeled hUC-MSCs were tracked by in vivo bioluminescence imaging (BLI), and human-specific DNA expression in wounds was detected by PCR. Inflammatory cells, neutrophils, macrophages, capillaries and collagen types I/III in wounds were evaluated by histochemical staining. Wound blood flow was evaluated by laser Doppler blood flow meter. The levels of proinflammatory and anti-inflammatory factors, VEGF, collagen types I/III in wounds were analyzed using an ELISA. RESULTS: We found that wound healing was significantly accelerated in the hUC-MSC therapy group. The hUC-MSCs migrated into wound and remarkably decreased the quantity of infiltrated inflammatory cells and levels of IL-1, IL-6, TNF-α and increased levels of IL-10 and TSG-6 in wounds. Additionally, the neovascularization and levels of VEGF in wounds in the hUC-MSC therapy group were markedly higher than those in other control groups. The ratio of collagen types I and III in the hUC-MSC therapy group were markedly higher than that in the burn group at indicated time after transplantation. CONCLUSION: The study suggests that hUC-MSCs transplantation can effectively improve wound healing in severe burned rat model. Moreover, these data might provide the theoretical foundation for the further clinical application of hUC-MSC in burn areas.
 

烧伤整形科

中国人民解放军总医院第四医学中心

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作者: 刘玲英

单位: 中国人民解放军总医院第四医学中心

来源: 中华医学会烧伤外科学分会2019年学术年会

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作者: 刘玲英

单位: 中国人民解放军总医院第四医学中心

来源: 中华医学会烧伤外科学分会2019年学术年会

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