标题：
Decreased Expression of KIFC1 in Human Testes with Globozoospermic Defects
讲者：
智二磊
单位：
上海市第一人民医院
播放：
1291
论文摘要：
Globozoospermia is a rare (prevalence of <0.1%) but severe male infertility condition.
In our previous study, we found that robust KIFC1 immunostaining was detected in the human
elongating/elongated spermatids during human acrosomogenesis. However, the relationship
between the decreased expression of KIFC1 and human globozoospermia remains largely unknown.
Testicular biopsies of 30 globozoospermia and 30 obstructive azoospermia patients who underwent
infertility evaluation and treatment were utilized in this study. Reverse transcriptase polymerase
chain reaction (RT-PCR), Western blots, immunohistochemistry, an in vivo model, and intratesticular
injection of small inhibitory RNA (siRNA) against the Kifc1 gene were employed, and sperm
abnormalities were evaluated by hematoxylin and eosin (H&E) staining and immunocytochemistry.
We revealed that the testicular level of KIFC1 mRNA in globozoospermia was significantly reduced
compared with that in obstructive azoospermia, and the KIFC1 protein was barely detectable in
testicular specimens in 30% (9 of 30) of patients with globozoospermia. Furthermore, knockdown
of the Kifc1 gene in mice increased the percentage of sperm with globozoospermic defects (26.5%).
Decreased KIFC1 expression was mainly observed in the testes of patients with globozoospermia at
the spermatid stage, which may be useful for counseling and management of such patients.
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